Journal: Journal of Pharmacy & Bioallied Sciences

Article Title: Cytotoxicity of Dhanwantharaarishtam on hiPSC Cells – An In vitro Study

doi: 10.4103/jpbs.jpbs_846_24

Figure Lengend Snippet: MTT assay of hiPSC cells treated with Dhanwantharaarishtam

Article Snippet: Human-induced pluripotent stem cells (hiPSCs) were only used for both cell viability and cytotoxic studies The hiPSC used in this study was obtained from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques: MTT Assay

Journal: Journal of Pharmacy & Bioallied Sciences

Article Title: Cytotoxicity of Dhanwantharaarishtam on hiPSC Cells – An In vitro Study

doi: 10.4103/jpbs.jpbs_846_24

Figure Lengend Snippet: Cytotoxicity assay of hiPSC cells treated with Dhanwantharaarishtam

Article Snippet: Human-induced pluripotent stem cells (hiPSCs) were only used for both cell viability and cytotoxic studies The hiPSC used in this study was obtained from National Centre for Cell Sciences (NCCS), Pune, India.

Techniques: Cytotoxicity Assay

Journal: Journal of Hepatocellular Carcinoma

Article Title: Coilin Affects the Prognosis of Hepatocellular Carcinoma Through Cell Cycle and Apoptosis

doi: 10.2147/JHC.S500119

Figure Lengend Snippet: Expression of COIL in HCC cell lines and HCC tissue from hospital. The expression of COIL in liver cancer cell lines huh7, hepG2 and hep3B was higher than that in L02 by immunohistochemistry ( A ) and Western blotting ( B ). ( C ) The relative expression of COIL in HCC was significantly higher than that in normal liver tissue by qRT-PCR (***p value < 0.001). ( D ) The expression of COIL in HCC tissue was higher than that in normal liver tissue. ( E, F ) Representative immunohistochemistry results of low or high COIL expression. ( G ) High COIL expression was associated with poor overall survival.

Article Snippet: The human normal liver cell line L02 was obtained from Beijing Baiao Bowei Biotechnology Co., Ltd.

Techniques: Expressing, Immunohistochemistry, Western Blot, Quantitative RT-PCR

Journal: The Journal of Liquid Biopsy

Article Title: Circulating epithelial cell as viral infection and tissue origin marker in patients with severe COVID-19

doi: 10.1016/j.jlb.2023.100005

Figure Lengend Snippet: Antibodies and fluorophores included in the study design, channels used for detection and cell lines used as controls.

Article Snippet: The hAELVi cell line was used as a positive control for RAGE protein (Rabbit polyclonal anti-RAGE-AF750, bs-0177R-A750, Bioss Inc), a specific lung tissue marker.

Techniques: Staining

Journal: EMBO Molecular Medicine

Article Title: A clinical and mechanistic study of topical borneol‐induced analgesia

doi: 10.15252/emmm.201607300

Figure Lengend Snippet: A–D Representative whole‐cell currents in HEK 293 cells expressing TRPV1 (A), ASIC3 (B), P2X2 (C), or P2X4 (D) in response to capsaicin (A), acidic pH (B), or ATP (C, D) in the absence of presence of borneol ( n = 5 for each channel). E The effect of different concentrations of borneol on the enzymatic activity of human COX‐2. The number of independent measurements is marked on top of each bar. F Averaged intracellular Ca 2+ increases in cultured mouse DRG neurons in response to consecutive applications of 200 μM borneol, 200 μM menthol, and 67 mM KCl. A total of 81 in 1,689 neurons from four mice were found to be borneol‐sensitive and were included in the analysis. G Representative intracellular Ca 2+ signals in HEK 293 cells expressing human TRPM8 (hTRPM8) in response to different concentrations of borneol. After each application of borneol, Ca 2+ ionophore ionomycin was applied to calibrate Ca 2+ response. RFU: relative fluorescence unit. H Dose–response curves of the borneol‐ or menthol‐induced increase in intracellular Ca 2+ in hTRPM8‐expressing HEK 293 cells. Smooth curves are fit to the Hill equation with an EC 50 of 65 μM and a Hill coefficient of 2.0 for borneol ( n = 15) and an EC 50 of 13 μM and a Hill coefficient of 2.0 for menthol ( n = 6 at concentrations of 0.1, 0.3, and 1 μM; n = 9 at concentration of 3 μM; n = 13 at concentrations of 10, 30, 100, and 300 μM). The data were normalized to ionomycin‐induced intracellular Ca 2+ increases. I Time course of menthol‐ and subsequently applied borneol‐induced whole‐cell currents in hTRPM8‐expressing HEK 293 cells ( n = 6). Data information: All the data are presented as the mean ± standard error of the mean (SEM).

Article Snippet: Intracellular calcium imaging of HEK 293 cells was performed using the FlexStation 3 microplate reader (Molecular Devices).

Techniques: Expressing, Activity Assay, Cell Culture, Fluorescence, Concentration Assay

Journal: EMBO Molecular Medicine

Article Title: A clinical and mechanistic study of topical borneol‐induced analgesia

doi: 10.15252/emmm.201607300

Figure Lengend Snippet: Representative intracellular Ca 2+ signals in HEK 293 cells transfected with empty vector in response to borneol and subsequent applied Ca 2+ ionophore ionomycin ( n = 6). Representative intracellular Ca 2+ signals in HEK 293 cells expressing hTRPM8 in response to different concentrations of menthol and the subsequently applied Ca 2+ ionophore ionomycin.

Article Snippet: Intracellular calcium imaging of HEK 293 cells was performed using the FlexStation 3 microplate reader (Molecular Devices).

Techniques: Transfection, Plasmid Preparation, Expressing

Journal: EMBO Molecular Medicine

Article Title: A clinical and mechanistic study of topical borneol‐induced analgesia

doi: 10.15252/emmm.201607300

Figure Lengend Snippet: Quantification of consecutively applied 100 μM menthol‐ and 600 μM borneol‐induced hTRPM8 currents. Currents were normalized to 100 μM menthol‐induced currents at +80 mV ( n = 6). Representative intracellular Ca 2+ signals in HEK 293 cells expressing mouse TRPM8 in response to different concentrations of borneol. Dose–response curves of borneol‐induced increase in intracellular Ca 2+ in mouse TRPM8‐expressing HEK 293 cells. The smooth curve is a fit to the Hill equation with an EC 50 of 116 μM ( n = 12). The data were normalized to ionomycin‐induced intracellular Ca 2+ increases. Time course of menthol‐ and subsequently applied borneol‐induced whole‐cell currents in mouse TRPM8‐expressing HEK 293 cells ( n = 5). Data information: All the data are presented as the mean ± standard error of the mean (SEM).

Article Snippet: Intracellular calcium imaging of HEK 293 cells was performed using the FlexStation 3 microplate reader (Molecular Devices).

Techniques: Expressing